T5525
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T5525
Sigma
3,3′,5,5′-Tetramethylbenzidine
tablet, 1 mg substrate per tablet
| Synonym: | TMB |
| CAS Number: | 54827-17-7 |
| Linear Formula: | [-C6H2(CH3)2-4-NH2]2 |
| Molecular Weight: | 240.34 |
| Beilstein Registry Number: | 2808541 |
| MDL number: | MFCD00007748 |
| PubChem Substance ID: | 24900293 |
Description
| Application | 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8 |
Properties
| form | tablet |
| mp | 168-171 °C(lit.) |
| storage temp. | 2-8°C |
Safety
References
| Cited Reference | 1. Holland, V.R., et al. Tet. 30, 3299, (1974) |
| 2. D.D. Garner et al. J. Forensic Sci. 21, 819, (1976) | |
| 3. J.C. Standefer, D. Vanderjagt Clin. Chem. 23, 749, (1977) | |
| 4. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94, 266, (1979) | |
| 5. Liem, H.H. et al. Anal. Biochem. 98, 388, (1979) | |
| 6. Bos, E.S., et al. J. Immunoassay 2, 187, (1981) | |
| 7. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127, 346, (1982) | |
| 8. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23, 453, (1985) | |
| reference | Aldrich MSDS 1, 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / FT-NMR 1 (1), 1236:D / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref, 693 / Structure Index 1, 222:D:3 |
